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primary antibodies include anti brca1  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc primary antibodies include anti brca1
    FIGURE 1 | Putative miR-15/107 targets within the <t>BRCA1</t> CDS. (A) Schematic representation of protein-coding BRCA1 transcript. (B) Density function and cumulative distribution of CDS length across all human coding mRNAs. (C) The rna22-predicted target site for the miR-15/107 group together with the corresponding miRNA:mRNA heteroduplexes.
    Primary Antibodies Include Anti Brca1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 449 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary antibodies include anti brca1/product/Cell Signaling Technology Inc
    Average 96 stars, based on 449 article reviews
    primary antibodies include anti brca1 - by Bioz Stars, 2026-03
    96/100 stars

    Images

    1) Product Images from "Post-transcriptional regulation of BRCA1 through its coding sequence by the miR-15/107 group of miRNAs."

    Article Title: Post-transcriptional regulation of BRCA1 through its coding sequence by the miR-15/107 group of miRNAs.

    Journal: Frontiers in genetics

    doi: 10.3389/fgene.2015.00242

    FIGURE 1 | Putative miR-15/107 targets within the BRCA1 CDS. (A) Schematic representation of protein-coding BRCA1 transcript. (B) Density function and cumulative distribution of CDS length across all human coding mRNAs. (C) The rna22-predicted target site for the miR-15/107 group together with the corresponding miRNA:mRNA heteroduplexes.
    Figure Legend Snippet: FIGURE 1 | Putative miR-15/107 targets within the BRCA1 CDS. (A) Schematic representation of protein-coding BRCA1 transcript. (B) Density function and cumulative distribution of CDS length across all human coding mRNAs. (C) The rna22-predicted target site for the miR-15/107 group together with the corresponding miRNA:mRNA heteroduplexes.

    Techniques Used:

    FIGURE 2 | Suppression of BRCA1 mRNA by miR-15/107 miRNAs. Cell lines transfected with miR-15/107 miRNA precursors show a significant decrease in the amount of available BRCA1 transcript 48 h post-transfection as detected by qRT-PCR. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 compared to control scramble miR by Student’s t-test, n = 3 in each group.
    Figure Legend Snippet: FIGURE 2 | Suppression of BRCA1 mRNA by miR-15/107 miRNAs. Cell lines transfected with miR-15/107 miRNA precursors show a significant decrease in the amount of available BRCA1 transcript 48 h post-transfection as detected by qRT-PCR. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 compared to control scramble miR by Student’s t-test, n = 3 in each group.

    Techniques Used: Transfection, Quantitative RT-PCR, Control

    FIGURE 4 | Sequestration of miR-15/107 miRNAs impacts BRCA1 mRNA. Cells transfected with luciferase reporter plasmids containing antisense (as) miRNA sequences or predicted wild type (WT) BRCA1 miR-15/107 MRE demonstrate varying levels of BRCA1 expression rescue. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 compared to control by Student’s t-test, n = 3 in each group.
    Figure Legend Snippet: FIGURE 4 | Sequestration of miR-15/107 miRNAs impacts BRCA1 mRNA. Cells transfected with luciferase reporter plasmids containing antisense (as) miRNA sequences or predicted wild type (WT) BRCA1 miR-15/107 MRE demonstrate varying levels of BRCA1 expression rescue. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 compared to control by Student’s t-test, n = 3 in each group.

    Techniques Used: Transfection, Luciferase, Expressing, Control

    FIGURE 5 | Translational suppression of BRCA1 by miR-15/107 miRNAs. Western immunoblots from hTERT-HPNE, HCT-116, and MIA PaCa-2 cells demonstrate relative levels of BRCA1 protein expression at 72 h post-transfection with control scramble miRNA and anti-miR precursors, miRNA and anti-miR precursors, or siBRCA1 control.
    Figure Legend Snippet: FIGURE 5 | Translational suppression of BRCA1 by miR-15/107 miRNAs. Western immunoblots from hTERT-HPNE, HCT-116, and MIA PaCa-2 cells demonstrate relative levels of BRCA1 protein expression at 72 h post-transfection with control scramble miRNA and anti-miR precursors, miRNA and anti-miR precursors, or siBRCA1 control.

    Techniques Used: Western Blot, Expressing, Transfection, Control



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    Cell Signaling Technology Inc primary antibodies include anti brca1
    FIGURE 1 | Putative miR-15/107 targets within the <t>BRCA1</t> CDS. (A) Schematic representation of protein-coding BRCA1 transcript. (B) Density function and cumulative distribution of CDS length across all human coding mRNAs. (C) The rna22-predicted target site for the miR-15/107 group together with the corresponding miRNA:mRNA heteroduplexes.
    Primary Antibodies Include Anti Brca1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary antibodies include anti brca1/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    primary antibodies include anti brca1 - by Bioz Stars, 2026-03
    96/100 stars
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    FIGURE 1 | Putative miR-15/107 targets within the BRCA1 CDS. (A) Schematic representation of protein-coding BRCA1 transcript. (B) Density function and cumulative distribution of CDS length across all human coding mRNAs. (C) The rna22-predicted target site for the miR-15/107 group together with the corresponding miRNA:mRNA heteroduplexes.

    Journal: Frontiers in genetics

    Article Title: Post-transcriptional regulation of BRCA1 through its coding sequence by the miR-15/107 group of miRNAs.

    doi: 10.3389/fgene.2015.00242

    Figure Lengend Snippet: FIGURE 1 | Putative miR-15/107 targets within the BRCA1 CDS. (A) Schematic representation of protein-coding BRCA1 transcript. (B) Density function and cumulative distribution of CDS length across all human coding mRNAs. (C) The rna22-predicted target site for the miR-15/107 group together with the corresponding miRNA:mRNA heteroduplexes.

    Article Snippet: Primary antibodies include anti-BRCA1 (D54A8, Cell Signaling, Danvers, MA, USA) and anti-β-actin (8H10D10, Cell Signaling).

    Techniques:

    FIGURE 2 | Suppression of BRCA1 mRNA by miR-15/107 miRNAs. Cell lines transfected with miR-15/107 miRNA precursors show a significant decrease in the amount of available BRCA1 transcript 48 h post-transfection as detected by qRT-PCR. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 compared to control scramble miR by Student’s t-test, n = 3 in each group.

    Journal: Frontiers in genetics

    Article Title: Post-transcriptional regulation of BRCA1 through its coding sequence by the miR-15/107 group of miRNAs.

    doi: 10.3389/fgene.2015.00242

    Figure Lengend Snippet: FIGURE 2 | Suppression of BRCA1 mRNA by miR-15/107 miRNAs. Cell lines transfected with miR-15/107 miRNA precursors show a significant decrease in the amount of available BRCA1 transcript 48 h post-transfection as detected by qRT-PCR. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 compared to control scramble miR by Student’s t-test, n = 3 in each group.

    Article Snippet: Primary antibodies include anti-BRCA1 (D54A8, Cell Signaling, Danvers, MA, USA) and anti-β-actin (8H10D10, Cell Signaling).

    Techniques: Transfection, Quantitative RT-PCR, Control

    FIGURE 4 | Sequestration of miR-15/107 miRNAs impacts BRCA1 mRNA. Cells transfected with luciferase reporter plasmids containing antisense (as) miRNA sequences or predicted wild type (WT) BRCA1 miR-15/107 MRE demonstrate varying levels of BRCA1 expression rescue. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 compared to control by Student’s t-test, n = 3 in each group.

    Journal: Frontiers in genetics

    Article Title: Post-transcriptional regulation of BRCA1 through its coding sequence by the miR-15/107 group of miRNAs.

    doi: 10.3389/fgene.2015.00242

    Figure Lengend Snippet: FIGURE 4 | Sequestration of miR-15/107 miRNAs impacts BRCA1 mRNA. Cells transfected with luciferase reporter plasmids containing antisense (as) miRNA sequences or predicted wild type (WT) BRCA1 miR-15/107 MRE demonstrate varying levels of BRCA1 expression rescue. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 compared to control by Student’s t-test, n = 3 in each group.

    Article Snippet: Primary antibodies include anti-BRCA1 (D54A8, Cell Signaling, Danvers, MA, USA) and anti-β-actin (8H10D10, Cell Signaling).

    Techniques: Transfection, Luciferase, Expressing, Control

    FIGURE 5 | Translational suppression of BRCA1 by miR-15/107 miRNAs. Western immunoblots from hTERT-HPNE, HCT-116, and MIA PaCa-2 cells demonstrate relative levels of BRCA1 protein expression at 72 h post-transfection with control scramble miRNA and anti-miR precursors, miRNA and anti-miR precursors, or siBRCA1 control.

    Journal: Frontiers in genetics

    Article Title: Post-transcriptional regulation of BRCA1 through its coding sequence by the miR-15/107 group of miRNAs.

    doi: 10.3389/fgene.2015.00242

    Figure Lengend Snippet: FIGURE 5 | Translational suppression of BRCA1 by miR-15/107 miRNAs. Western immunoblots from hTERT-HPNE, HCT-116, and MIA PaCa-2 cells demonstrate relative levels of BRCA1 protein expression at 72 h post-transfection with control scramble miRNA and anti-miR precursors, miRNA and anti-miR precursors, or siBRCA1 control.

    Article Snippet: Primary antibodies include anti-BRCA1 (D54A8, Cell Signaling, Danvers, MA, USA) and anti-β-actin (8H10D10, Cell Signaling).

    Techniques: Western Blot, Expressing, Transfection, Control